Methods Mix-cultured conical neurons of SD rats were subjected to mechanical scratch injury, and the degree of injury were assessed by cell count with trypan blue staining and by lactate dehydrogenase ( LDH) test. The intact cells were confirmed with neutral red counter staining. 方法采用机械划痕创伤性损伤培养大鼠皮层神经元,根据培养细胞锥虫篮活力计数和培养上清乳酸脱氢酶(LDH)检测评估损伤程度,用中性红复染确定未损伤细胞。